Comparison of reflectometric interference spectroscopy with other instruments for label-free optical detection

被引:0
|
作者
C. Hänel
G. Gauglitz
机构
[1] Institute of Physical and Theoretical Chemistry,
[2] University of Tübingen,undefined
[3] Auf der Morgenstelle 8,undefined
[4] 72076 Tübingen,undefined
[5] Germany,undefined
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Label-free detection Reflectometric interference spectroscopy Biomolecular interaction Thrombine Thrombine inhibitor;
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摘要
On the basis of kinetic measurements of biomolecular interactions, a reflectometric interference spectroscopy (RIfS) setup is compared with two commercial instruments. These instruments are based on evanescent wave techniques, surface plasmon resonance (SPR) (represented by BIAcore 2000) and resonant mirror (RM) technique (using IAsys plus). All methods allow a label-free and time-resolved optical detection of biomolecular interaction.
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页码:91 / 100
页数:9
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