Development and characterization of 42 novel polymorphic microsatellite markers for Odontobutis potamophila from EST sequences

被引：0

作者：

Lijuan Zhang

Hongwei Zhang

Yaping Zhang

Fei Zhu

Xinyuan Hou

Yali Hu

Shaowu Yin

机构：

[1] Nanjing Normal University,Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences

[2] Jiangsu Co-Innovation Center for Marine Bio-Industry Technology,undefined

来源：

Conservation Genetics Resources | 2014年 / 6卷

关键词：

Expressed sequence tags (EST); Microsatellites;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Expressed sequence tags (ESTs) are sources for microsatellite development. In this study, we isolated and characterized microsatellite makers for Odontobutis potamophila using next-generation sequencing. A total of 42 polymorphic microsatellite repeat markers were identified in 32 individuals from Dangtu wild population. The number of alleles per locus varied from 2 to 10, and the observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.061 to 0.878, respectively. These EST-SSR makers would provide sufficient polymorphism for population genetic studies, parentage and genome mapping of O. potamophila and its closely related fishes.

引用

页码：469 / 472

页数：3

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