Development and characterization of 42 novel polymorphic microsatellite markers for Odontobutis potamophila from EST sequences

被引：0
|
作者：
Lijuan Zhang
Hongwei Zhang
Yaping Zhang
Fei Zhu
Xinyuan Hou
Yali Hu
Shaowu Yin
机构：
[1] Nanjing Normal University,Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences
[2] Jiangsu Co-Innovation Center for Marine Bio-Industry Technology,undefined
来源：
Conservation Genetics Resources | 2014年 / 6卷
关键词：
Expressed sequence tags (EST); Microsatellites;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Expressed sequence tags (ESTs) are sources for microsatellite development. In this study, we isolated and characterized microsatellite makers for Odontobutis potamophila using next-generation sequencing. A total of 42 polymorphic microsatellite repeat markers were identified in 32 individuals from Dangtu wild population. The number of alleles per locus varied from 2 to 10, and the observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.061 to 0.878, respectively. These EST-SSR makers would provide sufficient polymorphism for population genetic studies, parentage and genome mapping of O. potamophila and its closely related fishes.
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页码：469 / 472
页数：3
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