Anaerobic Methane Oxidation in Enrichment Cultures from Deep Sediments of a Mud Volcano Peschanka (South Baikal)

被引:0
|
作者
A. V. Lomakina
E. V. Mamaeva
T. V. Pogodaeva
G. V. Kalmychkov
I. A. Khal’zov
T. I. Zemskaya
机构
[1] Russian Academy of Sciences,Limnological Institute, Siberian Branch
[2] Russian Academy of Sciences,Vinogradov Institute of Geochemistry, Siberian Branch
来源
Microbiology | 2018年 / 87卷
关键词
microbial consortia; anaerobic methane oxidation; ANME-2d archaea; NC10 bacteria; bottom sediments; Lake Baikal;
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摘要
Under laboratory conditions, the microbial communities of bottom sediments of a mud volcano Peschanka (Lake Baikal) were found to carry out anaerobic methane oxidation (AOM). After 16 days of anaerobic cultivation of the enrichment cultures, methane content in the gas phase decreased, and microbial consortia were established. The content of carbon, nitrogen, and oxygen determined by energy dispersive X-ray spectroscopy (EDS) was higher than in the nearby sediment particles. The presence of bacteria of the NC10 phylum and archaea of the ANME-2d cluster was established by fluorescent in situ hybridization (FISH).
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页码:317 / 325
页数:8
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