An In Vitro Assay Method for Resistance to Bacterial Wilt (Ralstonia solanacearum) in Potato

被引：0
|
作者：
Ippei Habe
机构：
[1] Nagasaki Agricultural and Forestry Technical Development Center,
来源：
American Journal of Potato Research | 2018年 / 95卷
关键词：
Phylotype I/biovar 4; Growth chamber; Controlled environment; Cultured plantlet;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
To develop an in vitro assay method for bacterial wilt resistance in potato, resistant and susceptible standard genotypes were grown in vitro, and different conditions of inoculation with Ralstonia solanacearum phylotype I/biovar 4 were examined. The optimal condition was the inoculation of 6–8 leaf stage plants with a bacterial concentration of 102 CFU ml−1 and an incubation temperature of 28 °C. Evaluation of stem wilting was more reliable than that of leaf wilting. Using this method, nine genotypes with different resistance levels in the field were evaluated. Lower disease indices were obtained for genotypes with high resistance levels in the field, suggesting that this assay is useful for evaluating bacterial wilt resistance in a controlled environment.
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页码：311 / 316
页数：5
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