Measurement of human serum albumin concentration using Raman spectroscopy setup

This work is dedicated to the measurement of plasma proteins concentration in liquid solutions using Raman spectroscopy setup. The obtained Raman spectra showed significant variation of intensities of certain spectral bands 940, 1005, 1330, 1450 and 1650 cm−1 for different protein fractions and concentrations. Partial least squares regression analysis was used for determination of correlation coefficients. It has been shown that the proposed method represents structure and biochemical composition of albumin and immunoglobulins A and G, and that albumin concentration may be measured with an accuracy of 6–8 g/L.