Thermoregulation of catalytic activity of enzymes polymeric derivatives

被引：0
|
作者：
I. L. Valuev
L. V. Vanchugova
Yu. A. Taluzhenkov
L. I. Valuev
T. A. Valueva
机构：
[1] Russian Academy of Sciences,Topchiev Institute of Petrochemical Synthesis
[2] Russian Academy of Sciences,Bakh Institute of Biochemistry
来源：
Applied Biochemistry and Microbiology | 2009年 / 45卷
关键词：
Trypsin; Apply Biochemistry; DEAA; Diblock Copolymer; Random Copolymer;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Trypsin was immobilized on polymeric carriers with low critical solution temperature (LCST). Homopolymer of N,N-diethylacrylamide (DEAA), random copolymers of DEAA and acrylamide (AA), and block copolymers poly-DEAA-poly-AA were used as the carriers. It was shown that at a temperature above LCST all carriers have a conformation change and trypsin’s polymeric derivatives precipitate. The maximal activity after phase transition keeps trypsin, immobilized on poly-DEAA block in poly-DEAA-poly-AA block-copolymer.
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页码：473 / 477
页数：4
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