Efficient plant regeneration of garlic (Allium sativum L.) By root-tip culture

被引：23
|
作者：
Robledo-Paz A. [1 ]
Villalobos-Arámbula V.M. [1 ]
Jofre-Garfias A.E. [1 ]
机构：
[1] Departamento de Ingeniería Genética de Plantas, Centro de Investigación y de Estudios Avanzados del I.P.N., 36500 Irapuato, Gto.
来源：
In Vitro Cellular & Developmental Biology - Plant | 2000年 / 36卷 / 5期
关键词：
Bulb induction; Callus induction; Organogenesis; Tissue culture;
D O I：
10.1007/s11627-000-0075-6
中图分类号：
学科分类号：
摘要：
Root apices from in vitro cultured garlic (Allium sativum) cloves of cvs. ABEN and GT96-1 were used as axenic explants for organogenic callus production and plant regeneration experiments. Explants cultured in media based on those of Chu and co-workers (N6) or Murashige and Skoog (MS) could induce organogenic callus after 8 wk culture in darkness. Both media were supplemented with 2,4-dichlorophenoxyacetic acid (2.2-4.5 μM), alone or combined with 6-furfurylaminopurine (kinetin, 2.3-4.6 μM). Shoots started to grow 3 wk after culturing in the presence of light and the addition to culture media of 4.4 μM N6-benzyladenine. Plants capable of producing microbulbs regenerated 6 wk later. Up to 170 plants g-1 FW callus were obtained when culturing was initiated in MS medium supplemented with 4.6 μM kinetin and 4.5 μM 2,4-dichlorophenoxyacetic acid.
引用
收藏
页码：416 / 419
页数：3
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