Gene expression profiling in single cells within tissue

被引：0

作者：

Capodieci P. ^{[1
]}

Donovan M. ^{[1
]}

Buchinsky H. ^{[1
]}

Jeffers Y. ^{[1
]}

Cordon-Cardo C. ^{[2
]}

Gerald W. ^{[2
]}

Edelson J. ^{[1
]}

Shenoy S.M. ^{[3
]}

Singer R.H. ^{[3
]}

机构：

[1] Aureon Laboratories, Yonkers, NY 10701

[2] Memorial Sloan Kettering Cancer Center, New York, NY 10021

[3] Albert Einstein College of Medicine, Bronx, NY 10461

来源：

Nature Methods | 2005年 / 2卷 / 9期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/nmeth786

中图分类号：

学科分类号：

摘要：

We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.

引用

页码：663 / 665

页数：2

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