Effects of auxin and light treatments of donor plants on shoot production from indica-type rice (Oryza sativa L.)

被引:11
|
作者
Padua V.L.M. [1 ,2 ]
Fernandes L.D. [3 ]
De Oliveira D.E. [1 ,2 ]
Mansur E. [4 ,5 ]
机构
[1] Universidade Federal do Rio de Janeiro, Instituto de Biologia, LGMV, Rio de Janeiro, RJ
[2] Universidade Federal do Rio de Janeiro, Instituto de Biologia, Departamento de Genetica, Rio de Janeiro, RJ
[3] Universidade Federal do Rio de Janeiro, Horto Botânico do Museu Nacional, Rio de Janeiro, RJ
[4] Universidade do Estado do Rio de Janeiro, IBRAG, Rio de Janeiro, RJ
[5] Universidade do Estado do Rio de Janeiro, Instituto de Biologia, LABMIT/DBCG, Rio de Janeiro, RJ
关键词
Donor plant; Micropropagation; Rice; Shoot apex;
D O I
10.1007/BF02822735
中图分类号
学科分类号
摘要
An efficient clonal propagation procedure for a Brazilian indica rice subspecies was developed with shoot apex explants. Shoot apices were excised from 4-d-old seedlings and cultured on MS medium supplemented with 8.9 μM 6-benzyladenine. The efficiency of shoot production was influenced by growth regulators and light treatments to the donor plant. Explants derived from seedlings grown in the presence of 10.7 μM naphthaleneacetic acid and in the absence of light showed significantly increased regeneration capacity as compared to control explants. Anatomical analysis of the new shoot meristems revealed that they originated from preexisting apical and axillary meristem as well as from the mesocotyl parenchyma.
引用
收藏
页码:285 / 288
页数:3
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