Characterization of ornamental Datura plants transformed by Agrobacterium rhizogenes

被引：26

作者：

Giovannini A. ^{[1
]}

Pecchioni N. ^{[2
]}

Rabaglio M. ^{[1
]}

Allavena A. ^{[1
]}

机构：

[1] Ist. Sperim. per la Floricoltura, 18038 Sanremo (IM), C.so Degli Inglesi

[2] Ist. Sperim. per la Cerealicoltura, 29017 Fiorenzuola d'Arda (PC), Via S. Protaso

来源：

In Vitro Cellular & Developmental Biology - Plant | 1997年 / 33卷 / 2期

关键词：

Brugmansia; Hairy roots; Ornamentals; Rol genes; Transgenic plants;

D O I：

10.1007/s11627-997-0004-z

中图分类号：

学科分类号：

摘要：

Datura arborea and D. sanguinea hairy roots were produced by cocultivation of leaf fragments with Agrobacterium rhizogenes strain NCPP 1855. Adventitious buds emerged spontaneously, without exogenous growth regulators, from seven hairy root clones of D. arborea and from one hairy root clone of D. sanguinea. Regenerated plants were successfully acclimatized in the greenhouse. The integration of the bacterial TL-DNA into the genome of the putative transformed plants was confirmed by Southern blot analysis. Transgenic plants displayed increased ability to root in vivo. Morphological traits with relevant ornamental value like plant height, leaf number, size and shape, internode number, and internode length were also affected. Transformation by wild-type Ri TL-DNA provided the chance to study plant growth and differentiation and to select improved genotypes.

引用

页码：101 / 106

页数：5

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