Keth-seq for transcriptome-wide RNA structure mapping

被引：0

作者：

Xiaocheng Weng

Jing Gong

Yi Chen

Tong Wu

Fang Wang

Shixi Yang

Yushu Yuan

Guanzheng Luo

Kai Chen

Lulu Hu

Honghui Ma

Pingluan Wang

Qiangfeng Cliff Zhang

Xiang Zhou

Chuan He

机构：

[1] Institute for Biophysical Dynamics,Department of Chemistry, Department of Biochemistry and Molecular Biology

[2] Howard Hughes Medical Institute,College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education

[3] The University of Chicago,MOE Key Laboratory of Bioinformatics, Beijing Advanced Innovation Center for Structural Biology, Center for Synthetic and Systems Biology, Tsinghua

[4] Wuhan University,Peking Joint Center for Life Sciences, School of Life Sciences

[5] Tsinghua University,undefined

[6] Wuhan University School of Pharmaceutical Sciences,undefined

来源：

Nature Chemical Biology | 2020年 / 16卷

关键词：

D O I：

暂无

中图分类号：

学科分类号：

摘要：

RNA secondary structure is critical to RNA regulation and function. We report a new N3-kethoxal reagent that allows fast and reversible labeling of single-stranded guanine bases in live cells. This N3-kethoxal-based chemistry allows efficient RNA labeling under mild conditions and transcriptome-wide RNA secondary structure mapping.

引用

页码：489 / 492

页数：3

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