Programmable RNA recognition and cleavage by CRISPR/Cas9

被引：0
|
作者：
Mitchell R. O’Connell
Benjamin L. Oakes
Samuel H. Sternberg
Alexandra East-Seletsky
Matias Kaplan
Jennifer A. Doudna
机构：
[1] University of California,Department of Molecular and Cell Biology
[2] University of California,Department of Chemistry
[3] Howard Hughes Medical Institute,Physical Biosciences Division
[4] University of California,undefined
[5] Lawrence Berkeley National Laboratory,undefined
[6] Present address: Department of Agricultural and Biological Engineering,undefined
[7] University of Florida,undefined
[8] Gainesville,undefined
[9] Florida 32611,undefined
[10] USA.,undefined
来源：
Nature | 2014年 / 516卷
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学科分类号：
摘要：
In the presence of a short DNA oligonucleotide containing a protospacer adjacent motif, a guide-RNA-programmed Cas9 is able to specifically bind and/or cleave single-stranded RNA—this system can be used to isolate specific endogenous RNA transcripts from a cell lysate without any tag or modification.
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页码：263 / 266
页数：3
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