A custom-made two-photon microscope and deconvolution system

被引：0
|
作者：
Ania Majewska
Glen Yiu
Rafael Yuste
机构：
[1] Department of Biological Sciences,
[2] Columbia University,undefined
[3] 1212 Amsterdam Avenue,undefined
[4] Box 2435,undefined
[5] New York,undefined
[6] NY 10027,undefined
[7] USA,undefined
来源：
Pflügers Archiv | 2000年 / 441卷
关键词：
Fluoview Laser Pockels Scanning Spines XCOSM;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We describe in detail a custom-built two-photon microscope based on a modified confocal scanhead (Olympus Fluoview) and mode-locked Ti:sapphire laser (Coherent Mira 900). This system has internal detectors as well as external whole-field detection and an electro-optical modulator for blanking the beam on flyback and effecting fast changes in excitation intensity. This microscope can be used in deep, scattering samples for quantitative measurements with a wide range of fluorophores (GFP, fura, calcium green, calcium orange, fluo-3, DiI, DiO, fluorescein, rhodamine), for fluorescent photobleaching recovery and for uncaging. Images obtained with this system can be deconvolved with the Estimation Maximization algorithm using the program XCOSM (freeware available at: http://www.ibc.wustl.edu/bcl/xcosm/).
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页码：398 / 408
页数：10
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