1H, 13C, and 15N NMR assignments of the Pyrococcus abyssi DNA polymerase II intein

被引：0
|
作者：
Jiajing Liu
Zhenming Du
Clayton D. Albracht
Roshni O. Naidu
Kenneth V. Mills
Chunyu Wang
机构：
[1] Center for Biotechnology and Interdisciplinary Studies,Biology Department
[2] Rensselaer Polytechnic Institute,Department of Chemistry
[3] College of the Holy Cross,undefined
来源：
Biomolecular NMR Assignments | 2011年 / 5卷
关键词：
Intein; NMR; Assignment; Hyperthermophile; DNA polymerase II;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Protein splicing is a precise post-translational process mediated by inteins. Inteins are intervening proteins that cleave themselves from a precursor protein while joining the flanking sequences. Here we report the 15N, 13C, and 1H chemical shift assignments of the intein from DNA polymerase II of Pyrococcus abyssi (Pab PolII intein), which has been recombinantly overexpressed and isotopically labeled. The NMR assignments of Pab PolII intein are essential for solution structure determination and protein dynamics study.
引用
收藏
相关论文
共 50 条
  • [41] 1H, 15N and 13C NMR assignments of mouse methionine sulfoxide reductase B2
    Åshild S. Breivik
    Finn L. Aachmann
    Lena S. Sal
    Hwa-Young Kim
    Rebecca Del Conte
    Vadim N. Gladyshev
    Alexander Dikiy
    Biomolecular NMR Assignments, 2009, 3 : 157 - 157
  • [42] 1H, 15N and 13C NMR assignments of mouse methionine sulfoxide reductase B2
    Breivik, Ashild S.
    Aachmann, Finn L.
    Sal, Lena S.
    Kim, Hwa-Young
    Del Conte, Rebecca
    Gladyshev, Vadim N.
    Dikiy, Alexander
    BIOMOLECULAR NMR ASSIGNMENTS, 2008, 2 (02) : 199 - 201
  • [43] 1H, 13C and 15N NMR assignments of a calcium-binding protein from Entamoeba histolytica
    Deepshikha Verma
    Alok Bhattacharya
    Kandala V. R. Chary
    Biomolecular NMR Assignments, 2016, 10 : 67 - 70
  • [44] 1H, 13C and 15N NMR Assignments for AlgH, a Putative Transcriptional Regulator from Pseudomonas Aeruginosa
    Aaron B. Cowley
    Ramona J. Bieber Urbauer
    Jeffrey L. Urbauer
    Journal of Biomolecular NMR, 2005, 33 : 74 - 74
  • [45] 1H, 13C and 15N NMR assignments of Duck HBV primer loop of the encapsidation signal epsilon
    van der Werf, R. M.
    Girard, F. C.
    Nelissen, F.
    Tessari, M.
    Wijmenga, S. S.
    BIOMOLECULAR NMR ASSIGNMENTS, 2008, 2 (02) : 143 - 145
  • [46] 1H, 13C, and 15N NMR assignments for the helicase interaction domain of Staphylococcus aureus DnaG primase
    Shortridge, Matthew D.
    Griep, Mark A.
    Powers, Robert
    BIOMOLECULAR NMR ASSIGNMENTS, 2012, 6 (01) : 35 - 38
  • [47] 1H, 13C and 15N NMR assignments of Duck HBV primer loop of the encapsidation signal epsilon
    R. M. van der Werf
    F. C. Girard
    F. Nelissen
    M. Tessari
    S. S. Wijmenga
    Biomolecular NMR Assignments, 2008, 2 : 143 - 145
  • [48] 1H, 13C and 15N NMR assignments for a carbon monoxide generating metalloenzyme from Klebsiella pneumoniae
    Mo, HP
    Dai, Y
    Pochapsky, SS
    Pochapsky, TC
    JOURNAL OF BIOMOLECULAR NMR, 1999, 14 (03) : 287 - 288
  • [49] 1H, 13C and 15N assignments of CdnL, an essential protein in Myxococcus xanthus
    Yasmina Mirassou
    Montserrat Elías-Arnanz
    S. Padmanabhan
    M. Angeles Jiménez
    Biomolecular NMR Assignments, 2013, 7 : 51 - 55
  • [50] 1H, 13C, and 15N NMR assignments for the helicase interaction domain of Staphylococcus aureus DnaG primase
    Matthew D. Shortridge
    Mark A. Griep
    Robert Powers
    Biomolecular NMR Assignments, 2012, 6 : 35 - 38
12345