Microgram-scale protein structure determination by NMR

被引：0
|
作者：
James M Aramini
Paolo Rossi
Clemens Anklin
Rong Xiao
Gaetano T Montelione
机构：
[1] Center for Advanced Biotechnology and Medicine,Department of Molecular Biology and Biochemistry
[2] and Northeast Structural Genomics Consortium (NESG),Department of Biochemistry and Molecular Biology
[3] Rutgers University,undefined
[4] Bruker BioSpin Corporation,undefined
[5] Robert Wood Johnson Medical School,undefined
来源：
Nature Methods | 2007年 / 4卷
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摘要：
Using conventional triple-resonance nuclear magnetic resonance (NMR) experiments with a 1 mm triple-resonance microcoil NMR probe, we determined near complete resonance assignments and three-dimensional (3D) structure of the 68-residue Methanosarcina mazei TRAM protein using only 72 μg (6 μl, 1.4 mM) of protein. This first example of a complete solution NMR structure determined using microgram quantities of protein demonstrates the utility of microcoil-probe NMR technologies for protein samples that can be produced in only limited quantities.
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页码：491 / 493
页数：2
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