Nanostring-based screening for tyrosine kinase fusions in inflammatory myofibroblastic tumors

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作者
Taisei Kurihara
Yoshiyuki Suehara
Keisuke Akaike
Takuo Hayashi
Shinji Kohsaka
Toshihide Ueno
Nobuhiko Hasegawa
Tatsuya Takagi
Keita Sasa
Taketo Okubo
Youngji Kim
Hiroyuki Mano
Takashi Yao
Kazuo Kaneko
Tsuyoshi Saito
机构
[1] Juntendo University,Department of Human Pathology, Graduate School of Medicine
[2] Juntendo University School of Medicine,Department of Orthopedic Surgery
[3] Juntendo University,Intractable Disease Research Center, Graduate School of Medicine
[4] National Cancer Center Research Institute,Division of Cellular Signaling
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Scientific Reports | / 10卷
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摘要
Gene expression imbalances were measured for tyrosine kinase (TK) genes using Nanostring in 19 samples of inflammatory myofibroblastic tumor (IMT). All cases were immunohistochemically stained with anaplastic lymphoma kinase (ALK) and pan-tropomyosin-related-kinase (pan-Trk) antibodies. Five cases with imbalanced ALK expression, reported with Nanostring, were tested using fluorescence in situ hybridization (FISH); two cases with imbalanced neurotrophic tyrosine receptor kinase 3 (NTRK3) expression were tested using reverse transcription-polymerase chain reaction (RT-PCR). One case with imbalanced expression for ROS proto-oncogene 1 (ROS1) was tested using RNA sequencing and RT-PCR. TK fusions were detected in all cases with imbalanced TK expression. RNA sequencing detected a FN1–ROS1 fusion gene in an adult IMT case. IMT with ALK rearrangement showed myofibroblast-dominant features. IMT with ETV6–NTRK3 fusion showed prominent lymphoplasmacytic infiltration with scattered myofibroblasts. Pan-Trk IHC revealed only scattered positively stained cells in IMT with ETV6–NTRK3 fusion gene. ROS1-positive IMT showed myofibroblast-dominant features.
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