Protein-Metal Interactions Probed by SERS: Lysozyme on Nanostructured Gold Surface

被引:0
|
作者
N. R. Agarwal
M. Tommasini
E. Ciusani
A. Lucotti
S. Trusso
P. M. Ossi
机构
[1] Politecnico di Milano,Dipartimento di Chimica, Materiali e Ingegneria Chimica “Giulio Natta”
[2] McMaster University,Biointerfaces Institute and Department of Chemistry and Chemical Biology
[3] Istituto Nazionale Neurologico “Carlo Besta”,Dipartimento di Energia & Centre for NanoEngineered MAterials and Surfaces—NEMAS
[4] CNR-IPCF,undefined
[5] Istituto per i Processi Chimico-Fisici del CNR,undefined
[6] Politecnico di Milano,undefined
来源
Plasmonics | 2018年 / 13卷
关键词
Surface-enhanced Raman scattering; Label-free detection; Laser ablation; Nanostructured substrates; Lysozyme; Protein binding;
D O I
暂无
中图分类号
学科分类号
摘要
Surface-enhanced Raman scattering is a well-established technique for molecular detection at low concentration, which is becoming increasingly popular in the field of biotechnology and health sciences. Since the process is understood in depth, the technique is becoming reliable. In this contribution, we consider another aspect of SERS besides molecular detection, focusing on the binding mechanisms of a complex system such as a protein to the noble metal substrates required by the technique itself. We also show that using a solid nanostructured substrate produced by controlled pulsed laser deposition SERS enables label-free detection of a protein. This is checked on lysozyme as a well-known prototype. Use of solid substrates with controlled morphology proves advantageous over colloidal systems for SERS applications. Moreover, such substrates are superior in terms of shelf life, packaging and ease of shipment.
引用
收藏
页码:2117 / 2124
页数:7
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