Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, Macrobrachium rosenbergii

被引：0
|
作者：
L. M. See
R. Hassan
S. G. Tan
S. Bhassu
机构：
[1] University Putra Malaysia,Biology Department, Faculty of Science
[2] Freshwater Fisheries Research Center,Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences
[3] University Putra Malaysia,Department of Genetics, Institute of Biological Sciences
[4] University Malaya,undefined
[5] Center for Biotechnology in Agriculture (CEBAR),undefined
来源：
Russian Journal of Genetics | 2011年 / 47卷
关键词：
Freshwater Prawn; Macrobrachium Rosenbergii; Giant Freshwater Prawn; Western Form; Freshwater Prawn Macrobrachium Rosenbergii;
D O I：
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中图分类号：
学科分类号：
摘要：
Seven single locus microsatellite markers were characterized in Malaysian giant freshwater prawn, Macrobrachium rosenbergii from an enriched genomic library Primer pairs were designed to flank the repeat sequences and the loci characterized for this species. The bands resulting from the PCR amplifications of these eight microsatellite loci were polymorphic with the number of alleles ranging from 8 to 26 alleles per locus, whereas the observed heterozygosity ranged from 0.0641 to 0.6564. These newly developed microsatellite markers should prove to be useful for population studies and in the management of genetic variations in broodstocks of freshwater prawn, M. rosenbergii.
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页码：497 / 499
页数：2
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