Isolation and characterization of microsatellite loci in the Malaysian giant freshwater prawn, Macrobrachium rosenbergii

被引：4
|
作者：
Bhassu, S. [1 ]
See, L. M. [2 ]
Hassan, R. [1 ]
Siraj, S. [2 ]
Tan, S. G. [2 ]
机构：
[1] Freshwater Fisheries Res Ctr, Titi 71650, Jelebu, Malaysia
[2] Univ Putra Malaysia, Fac Sci, Dept Biol, Selangor 43400, Malaysia
来源：
MOLECULAR ECOLOGY RESOURCES | 2008年 / 8卷 / 05期
关键词：
5 '-anchored polymerase chain reaction; heterozygosity; Macrobrachium rosenbergii; microsatellite loci; polymorphisms; RAMs;
D O I：
10.1111/j.1755-0998.2008.02127.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Eight single locus microsatellite markers were developed to characterize the Malaysian giant freshwater prawn, Macrobrachium rosenbergii. These microsatellites were isolated from an enriched genomic library contained by using a 5'-anchored polymerase chain reaction technique. Primers were designed to flank the repeat sequences and subsequently used to characterize 30 unrelated individuals of the giant freshwater prawn. The polymerase chain reaction amplification products of these eight microsatellite loci were polymorphic with the number of alleles ranging from two to 10 alleles per locus while the levels of heterozygosity ranged from 0.6333 to 0.8667.
引用
收藏
页码：983 / 985
页数：3
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