Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA

被引:0
|
作者
Christopher D Richardson
Graham J Ray
Mark A DeWitt
Gemma L Curie
Jacob E Corn
机构
[1] Innovative Genomics Initiative,Department of Molecular and Cell Biology
[2] University of California,undefined
[3] Berkeley,undefined
[4] University of California,undefined
[5] Berkeley,undefined
来源
Nature Biotechnology | 2016年 / 34卷
关键词
D O I
暂无
中图分类号
学科分类号
摘要
The efficiency of homology-directed genome editing with CRISPR-Cas9 is boosted through improved design of donor DNA.
引用
收藏
页码:339 / 344
页数:5
相关论文
共 50 条
  • [41] Targeted Genome Editing of Virulent Phages Using CRISPR-Cas9
    Lemay, Marie-Laurence
    Renaud, Ariane C.
    Rousseau, Genevieve M.
    Moineau, Sylvain
    BIO-PROTOCOL, 2018, 8 (01):
  • [42] Transient Manipulation of DNA Damage Repair Pathway Choice Improves Homology-Directed Repair During CRISPR/Cas9-Mediated Genome Editing
    Mandal, Pankaj K.
    Paulsen, Bruna S.
    Frock, Richard
    Gutierrez-Martinez, Paula
    Ebina, Wataru
    Agarwal, Suneet
    Alt, Frederick
    Rossi, Derrick J.
    MOLECULAR THERAPY, 2016, 24 : S227 - S227
  • [43] Conditional Genome Editing in the Mammalian Brain Using CRISPR-Cas9
    Haojie Sun
    Jie Zheng
    Ming Yi
    You Wan
    Neuroscience Bulletin, 2021, 37 : 423 - 426
  • [44] Highly efficient genome editing by homology-directed repair using Cas9 protein in Ceratitis capitata
    Aumann, Roswitha A.
    Schetelig, Marc F.
    Haecker, Irina
    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2018, 101 : 85 - 93
  • [45] Generation of chromosomal translocations that lead to conditional fusion protein expression using CRISPR-Cas9 and homology-directed repair
    Vanoli, Fabio
    Jasin, Maria
    METHODS, 2017, 121 : 138 - 145
  • [46] Rapid DNA unwinding accelerates genome editing by engineered CRISPR-Cas9
    Eggers, Amy R.
    Chen, Kai
    Soczek, Katarzyna M.
    Tuck, Owen T.
    Doherty, Erin E.
    Xu, Bryant
    Trinidad, Marena I.
    Thornton, Brittney W.
    Yoon, Peter H.
    Doudna, Jennifer A.
    CELL, 2024, 187 (13) : 3249 - 3261.e14
  • [47] DNA-Free Genome Editing in Hexaploid Sweetpotato Directed by Preassembled CRISPR-Cas9 Ribonucleoprotein Complexes
    Adrianne, Brown
    Egnin, M.
    Bukari, F.
    Mortley, D.
    Bonsi, C.
    Idehen, O.
    Alexander, D.
    Bernard, G.
    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 2022, 58 (04) : 676 - 676
  • [48] Enhancing Crop Resilience to Drought Stress through CRISPR-Cas9 Genome Editing
    Rai, Gyanendra Kumar
    Khanday, Danish Mushtaq
    Kumar, Pradeep
    Magotra, Isha
    Choudhary, Sadiya M.
    Kosser, Rafia
    Kalunke, Raviraj
    Giordano, Maria
    Corrado, Giandomenico
    Rouphael, Youssef
    Pandey, Sudhakar
    PLANTS-BASEL, 2023, 12 (12):
  • [49] CRISPR/Cas9 Ribonucleoprotein Nucleofection for Genome Editing in Primary Human Keratinocytes: Knockouts, Deletions, and Homology-Directed Repair Mutagenesis
    Bamundo, Martina
    Palumbo, Sara
    D'Auria, Ludovica
    Missero, Caterina
    Di Girolamo, Daniela
    CURRENT PROTOCOLS, 2024, 4 (11):
  • [50] Topoisomerase Inhibitors and PIM1 Kinase Inhibitors Improve Gene Editing Efficiency Mediated by CRISPR-Cas9 and Homology-Directed Repair
    Yun, Ying
    Wang, Min
    Guo, Shimeng
    Xie, Xin
    MOLECULES, 2024, 29 (12):