Automated live cell imaging of cell migration across a microfluidic-controlled chemoattractant gradient

被引：0
|
作者：
Philip Lee
Cindy S Y Chen
Terry Gaige
Paul J Hung
机构：
[1] EMD Millipore,
来源：
Nature Methods | 2015年 / 12卷
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摘要：
The quantification of cell-migration behavior in response to gradients is critical for understanding cellular movement and development. The CellASIC® ONIX microfluidic platform offers a microfluidic gradient plate to enable precision-controlled chemoattractant diffusion across perfusion barriers, enabling a quantitatively defined diffusion gradient stable enough for long-term, live cell analysis. Using the system, we studied the effect of a serum gradient on metastatic breast cancer cell migration distance, velocity and degree of chemotaxis.
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页码：vii / viii
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