Automated live cell imaging of cell migration across a microfluidic-controlled chemoattractant gradient

被引：6
|
作者：
Lee, Philip [1 ]
Chen, Cindy S. Y. [1 ]
Gaige, Terry [1 ]
Hung, Paul J. [1 ]
机构：
[1] EMD Millipore, Billerica, MA USA
来源：
NATURE METHODS | 2015年 / 12卷 / 11期
关键词：
N-CADHERIN;
D O I：
10.1038/nmeth.f.387
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The quantification of cell-migration behavior in response to gradients is critical for understanding cellular movement and development. The CellASIC® ONIX microfluidic platform offers a microfluidic gradient plate to enable precision-controlled chemoattractant diffusion across perfusion barriers, enabling a quantitatively defined diffusion gradient stable enough for long-term, live cell analysis. Using the system, we studied the effect of a serum gradient on metastatic breast cancer cell migration distance, velocity and degree of chemotaxis.
引用
收藏
页码：vii / viii
页数：2
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