Octaarginine-modified multifunctional envelope-type nanoparticles for gene delivery

被引：0
|
作者：
I A Khalil
K Kogure
S Futaki
S Hama
H Akita
M Ueno
H Kishida
M Kudoh
Y Mishina
K Kataoka
M Yamada
H Harashima
机构：
[1] Graduate School of Pharmaceutical Sciences,
[2] Hokkaido University,undefined
[3] Kita-ku,undefined
[4] Sapporo,undefined
[5] Hokkaido,undefined
[6] Japan,undefined
[7] The Core Research for Evolutional Science and Technology (CREST),undefined
[8] Precursory Research for Embryonic Science and Technology,undefined
[9] Japan Science and Technology Agency (JST),undefined
[10] Shibuya,undefined
[11] Institute for Chemical Research,undefined
[12] Kyoto University,undefined
[13] Faculty of Pharmaceutical Sciences,undefined
[14] Toyama Medical and Pharmaceutical University,undefined
[15] Sugitani,undefined
[16] Yamada Research Unit,undefined
[17] RIKEN Brain Science Institute,undefined
[18] National Institute of Environmental Health,undefined
[19] Research Triangle Park,undefined
[20] Graduate School of Engineering,undefined
[21] The University of Tokyo,undefined
[22] Hongo,undefined
来源：
Gene Therapy | 2007年 / 14卷
关键词：
non-viral gene delivery system; programmed packaging; octaarginine; multifunctional envelope-type nano device; topical application; hair growth;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
This study describes a multifunctional envelope-type nano device (MEND) that mimics an envelope-type virus based on a novel packaging strategy. MEND particles contain a DNA core packaged into a lipid envelope modified with an octaarginine peptide. The peptide mediates internalization via macropinocytosis, which avoids lysosomal degradation. MEND-mediated transfection of a luciferase expression plasmid achieved comparable efficiency to adenovirus-mediated transfection, with lower associated cytotoxicity. Furthermore, topical application of MEND particles containing constitutively active bone morphogenetic protein (BMP) type IA receptor (caBmpr1a) gene had a significant impact on hair growth in vivo. These data demonstrate that MEND is a promising non-viral gene delivery system that may provide superior results to existing non-viral gene delivery technologies.
引用
收藏
页码：682 / 689
页数：7
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