Specific binding of IL-8 to rabbit α-macroglobulin modulates IL-8 function in the lung

被引：0
|
作者：
A. Kurdowska
N. Fujisawa
B. Peterson
F.K. Carr
J.M. Noble
S.M. Alden
E.J. Miller
M. Teodorescu
机构：
[1] Department of Biochemistry,
[2] University of Texas Health Center,undefined
[3] Tyler,undefined
[4] TX 75708,undefined
[5] USA,undefined
[6] Fax: 903-877-5101,undefined
[7] e-mail: anna.kurdowska@uthct.edu.,undefined
[8] Department of Cell Biology,undefined
[9] University of Texas,undefined
[10] Health Center,undefined
[11] Tyler,undefined
[12] TX 75708,undefined
[13] USA,undefined
[14] Department of Microbiology and Immunology,undefined
[15] College of Medicine,undefined
[16] University of Illinois,undefined
[17] Chicago,undefined
[18] IL 60612,undefined
[19] USA,undefined
来源：
Inflammation Research | 2000年 / 49卷
关键词：
Key words: Neutrophils – Chemokines – Carrier proteins –α2-Macroglobulin receptors – Alveolar macrophages;
D O I：
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中图分类号：
学科分类号：
摘要：
Objective and Design: The purpose of this study was to compare chemotactic activity of IL-8 alone with that of IL-8 reacted with rabbit α-macroglobulins (αM) in vivo.¶Methods: Initially the binding of recombinant human IL-8 (rhIL-8) to rabbit αM was studied. 125I-labeled rhIL-8 was incubated with αM, and electrophoresed on native 5% gels or SDS-polyacrylamide 4-20% gradient gels. Next, rhIL-8 or rhIL-8 bound to αM was administered via an endotracheal tube to rabbit's lungs.¶Treatment: An endotracheal tube was wedged into a segment of the lobe of each lung, and a sample instilled through the tube into this segment. After 4 h the lungs were lavaged.¶Results: rhIL-8 bound to αM retained its full chemotactic activity in vitro but exhibited a diminished ability to induce the influx of neutrophils into the rabbit lung.¶Conclusions: The data suggest that αM may facilitate IL-8 clearance from the lung.
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页码：591 / 599
页数：8
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