Tetranucleotide microsatellite markers in Cryptomeria japonica D. Don

被引:0
|
作者
L. Guan
S. Shiraishi
机构
[1] Kyushu University,Graduate School of Bioresource and Bioenvironmental Sciences
[2] Kyushu University,Faculty of Agriculture
来源
关键词
Japanese cedar; Tetranucleotide microsatellite; Suppression PCR;
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学科分类号
摘要
Japanese cedar (Cryptomeria japonica D. Don) is an important ecological and commercial species in Japan. We isolated 11 polymorphic tetranucleotide microsatellite markers by using a ligation-mediated suppression PCR technique for Japanese cedar and investigated the polymorphism of these markers in 24 individuals from natural populations. The number of alleles ranged from 6 to 20 per locus, and expected heterozygosities of the 11 loci ranged from 0.664 to 0.950. These polymorphic markers will be powerful tools for the studies of individual genotyping, population genetics, and conservation genetics.
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页码:283 / 285
页数:2
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