In Silico screening for microsatellite markers from expressed sequence tags of Porphyra yezoensis (Bangiales, Rhodophyta)

被引：8
|
作者：
Wang M. [1 ]
Hu J. [1 ]
Zhuang Y. [1 ]
Zhang L. [1 ]
Liu W. [1 ]
Mao Y. [1 ]
机构：
[1] College of Marine Life Science, Ocean University of China
来源：
Journal of Ocean University of China | 2007年 / 6卷 / 2期
关键词：
Bioinformatics data mining; EST; Microsatellites; Porphyra yezoensis;
D O I：
10.1007/s11802-007-0161-z
中图分类号：
学科分类号：
摘要：
The genomic resources of Porphyra yezoensis expressed sequence tags (ESTs) were utilized to identify simple sequence repeats (SSRs), or microsatellites. This method took the advantage of using ESTs and microsatellites either for the establishment of gene identities or for the acquisition of high polymorphism. The microsatellites can be used as gene markers when microsatellites are tagged to genes. Revealed by bioinformatics analysis, 1162 out of 21 954 ESTs contained microsatellites and cluster analysis indicated that 984 of these ESTs fell into 112 contigs, while the other 178 ESTs were singletons. A total of 290 unique SSR-containing genes were identified. The AAC SSRs were the most populous type of microsatellites. GC-rich microsatellites were predominant among all the microsatellites. © Science Press 2007.
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页码：161 / 166
页数：5
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