High-resolution mass spectrometry of small molecules bound to membrane proteins

被引：0

作者：

Gault J. ^{[1
]}

Donlan J.A.C. ^{[1
]}

Liko I. ^{[1
]}

Hopper J.T.S. ^{[1
]}

Gupta K. ^{[1
]}

Housden N.G. ^{[2
]}

Struwe W.B. ^{[1
]}

Marty M.T. ^{[1
]}

Mize T. ^{[1
]}

Bechara C. ^{[1
,5
]}

Zhu Y. ^{[3
]}

Wu B. ^{[3
]}

Kleanthous C. ^{[2
]}

Belov M. ^{[4
]}

Damoc E. ^{[4
]}

Makarov A. ^{[4
]}

Robinson C.V. ^{[1
]}

机构：

[1] Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford

[2] Department of Biochemistry, University of Oxford, Oxford

[3] CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Pudong

[4] Thermo Fisher Scientific, Bremen

[5] UMR 5235 CNRS, Université de Montpellier, Montpellier

来源：

Nature Methods | 2016年 / 13卷 / 4期

基金：

英国惠康基金; 英国生物技术与生命科学研究理事会; 英国医学研究理事会;

关键词：

D O I：

10.1038/nmeth.3771

中图分类号：

学科分类号：

摘要：

Small molecules are known to stabilize membrane proteins and to modulate their function and oligomeric state, but such interactions are often hard to precisely define. Here we develop and apply a high-resolution, Orbitrap mass spectrometry-based method for analyzing intact membrane protein-ligand complexes. Using this platform, we resolve the complexity of multiple binding events, quantify small molecule binding and reveal selectivity for endogenous lipids that differ only in acyl chain length. © 2016 Nature America, Inc. All rights reserved.

引用

页码：333 / 336

页数：3

共 50 条

[41] WEIGHTED NMF FOR HIGH-RESOLUTION MASS SPECTROMETRY ANALYSIS
Dubroca, Remi
Junot, Christophe
Souloumiac, Antoine
2012 PROCEEDINGS OF THE 20TH EUROPEAN SIGNAL PROCESSING CONFERENCE (EUSIPCO), 2012, : 1806 - 1810
[42] Quantitation of acrylamide in foods by high-resolution mass spectrometry
Fogliano, Vincenzo (vincenzo.fogliano@wur.nl), 1600, American Chemical Society (62):
[43] ANALYTICAL APPLICATIONS OF HIGH-RESOLUTION MASS-SPECTROMETRY
ABRAMSON, FP
JOURNAL OF CHEMICAL EDUCATION, 1972, 49 (05) : A283 - &
[44] Analysis of trimethylgallium decomposition by high-resolution mass spectrometry
Ye, Zheng
Nitta, Shugo
Honda, Yoshio
Pristovsek, Markus
Amano, Hiroshi
Japanese Journal of Applied Physics, 2020, 59 (02):
[45] An insight into high-resolution mass-spectrometry data
Eckel-Passow, J. E.
Oberg, A. L.
Therneau, T. M.
Bergen, H. R., III
BIOSTATISTICS, 2009, 10 (03) : 481 - 500
[46] Determination of Phosphatidylcholine in Shrimp by High-Resolution Mass Spectrometry
Zhou, Li
Wang, Yan
Wang, Xiaolin
Liang, Yi
Huang, Zheng
ANALYTICAL LETTERS, 2019, 52 (02) : 308 - 319
[47] Proteotyping of the Parainfluenza Virus with High-Resolution Mass Spectrometry
Nguyen, An P.
Downard, Kevin M.
ANALYTICAL CHEMISTRY, 2013, 85 (02) : 1097 - 1105
[48] THE APPLICATION OF MAXENT TO HIGH-RESOLUTION MASS-SPECTROMETRY
FERRIGE, AG
SEDDON, MJ
SKILLING, J
ORDSMITH, N
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1992, 6 (12) : 765 - 770
[49] Signal detection in high-resolution mass spectrometry data
McLerran, Dale F.
Feng, Ziding
Semmes, O. John
Cazares, Lisa
Randolph, Timothy W.
JOURNAL OF PROTEOME RESEARCH, 2008, 7 (01) : 276 - 285
[50] High-resolution mass spectrometry for thymosins detection and characterization
Cabras, Tiziana
Iavarone, Federica
Martelli, Claudia
Delfino, Daniela
Rossetti, Diana Valeria
Inserra, Ilaria
Manconi, Barbara
Desiderio, Claudia
Messana, Irene
Hannappel, Ewald
Faa, Gavino
Castagnola, Massimo
EXPERT OPINION ON BIOLOGICAL THERAPY, 2015, 15 : S191 - S201

← 1 2 3 4 5 →