A cyclin-dependent kinase inhibitor (p21WAF1/CIP1) affects thymidine incorporation in human liver cancer cells

被引：0
|
作者：
Y Gong
S Deng
M Zhang
G Wang
G Y Minuk
F Burczynski
机构：
[1] Faculty of Medicine,Department of Internal Medicine
[2] University of Manitoba,Department of Biochemistry and Medical Genetics
[3] Faculty of Medicine,undefined
[4] University of Manitoba,undefined
[5] Faculty of Pharmacy,undefined
[6] University of Manitoba,undefined
来源：
British Journal of Cancer | 2002年 / 86卷
关键词：
p21; [; H]thymidine incorporation; liver cancer;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
p21WAF1/CIP1 is a universal cyclin-dependent kinase inhibitor. To investigate the role of p21WAF1/CIP1 in proliferation of human liver cancer cells, we examined the expression of p53, p21WAF1/CIP1, cdk2 and cdk4 expression in two human liver cancer cell lines (HepG2 and PLC/PRF/5 cells). The effects of p21WAF1/CIP1 on [3H]thymidine incorporation and cdks were also examined in these cells. HepG2 cells expressed all these proteins with lower level of cdk4. PLC/PRF/5 cells expressed the other proteins except p21WAF1/CIP1. Transfection of p21WAF1/CIP1 gene inhibited [3H]thymidine incorporation of both cells with different extent. Although the transfection of p21WAF1/CIP1 did not affect cdk2 and cdk4 expression, it did reduce cdk2 kinase activity by 20%. These results suggest that: (a) p21WAF1/CIP1 involved in DNA synthesis of human liver cancer cells; (b) p21WAF1/CIP1 could be a target gene for the treatment of human hepatocellular carcinoma.
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页码：625 / 629
页数：4
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