Coverage recommendations for methylation analysis by whole-genome bisulfite sequencing

被引：0

作者：

Ziller M.J. ^{[1
,2
,3
]}

Hansen K.D. ^{[4
,5
]}

Meissner A. ^{[1
,2
,3
]}

Aryee M.J. ^{[1
,6
,7
,8
]}

机构：

[1] Broad Institute of MIT and Harvard, Cambridge, MA

[2] Harvard Stem Cell Institute, Cambridge, MA

[3] Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA

[4] McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins School of Medicine, Baltimore, MD

[5] Department of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD

[6] Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA

[7] Center for Cancer Research, Massachusetts General Hospital, Charlestown, MA

[8] Department of Pathology, Harvard Medical School, Boston, MA

来源：

Nature Methods | 2015年 / 12卷 / 3期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/nmeth.3152

中图分类号：

学科分类号：

摘要：

Whole-genome bisulfite sequencing (WGBS) allows genome-wide DNA methylation profiling, but the associated high sequencing costs continue to limit its widespread application. We used several high-coverage reference data sets to experimentally determine minimal sequencing requirements. We present data-derived recommendations for minimum sequencing depth for WGBS libraries, highlight what is gained with increasing coverage and discuss the trade-off between sequencing depth and number of assayed replicates. © 2015 Nature America, Inc.

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页码：230 / 232

页数：2

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