Genetic polymorphism of Gentiana lutea L. (Gentianaceae) populations from the Chornohora Ridge of the Ukrainian Carpathians

Features of genetic structure and level of diversity of G. lutea populations from the Chornohora Ridge of the Ukrainian Carpathians were studied using RAPD- and ISSR-PCR. A high level of genetic diversity for studied populations concerning these DNA-markers is shown. The differences between populations are equal to 59–72% of the total genetic variation, whereas intrapopulation polymorphism makes up 28–41%. We assume that the relationships between genetic variability level and ecological-geographical conditions, as well as biological features of the species, are possible. The obtained results indicate the genetic isolation of G. lutea Chornohora populations from the Ukrainian Carpathians. Pozhyzhevska Mountain agropopulation was characterized by high level of polymorphism that means the possibility to use artificial plantings of this species for its preservation.