Synthesis and Evaluation of Antimicrobial Silver Nanoparticles on Multidrug-Resistant Bacterial Isolates from Urine Samples of Diabetic Patients and Infected Human Soft Tissues

Antimicrobial citrate-stabilized silver nanoparticles (AgNPs) were synthesized by dissolving silver nitrate in water and the antimicrobial activity was studied against multidrug-resistant bacterial isolates from urine samples of diabetic patients and infected human soft tissues of patients undergoing prolonged antibiotic treatment. The minimum inhibitory concentration and minimum bactericidal concentration values of the AgNPs were found to be 60, 80 and 140, 160 μg/mL against Bacillus megaterium and Staphyloccus aureus, respectively, isolated from infected human soft tissues and 60, 80 and 120, 160 μg/mL against Kluyvera ascorbata and Escherichia coli, respectively, isolated from urine samples of diabetic patients. The sizes of the AgNPs were found to be of 141 nm and were stable till 120 days at 37 °C. The AgNPs were found to be effective against both gram-positive and negative bacterial isolates.