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Pedobacter paludis sp. nov., isolated from wetland soil
被引:0
|作者:
Yuxiao Zhang
Jingxin Li
Jinling Wang
Gejiao Wang
机构:
[1] Huazhong Agricultural University,State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology
[2] Huazhong Agricultural University,College of Basic Science
来源:
关键词:
Mining soil;
Polyphasic taxonomy;
16S rRNA gene;
Genome;
D O I:
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学科分类号:
摘要:
A Gram-stain-negative, aerobic, non-motile, rod-shaped and non-spore-forming bacterium, designated YXT, was isolated from wetland soil. Compared to strain YXT, Pedobacter yonginense HMD1002T had the highest 16S rRNA gene sequence identity (97.8%), and the remaining strains had the identities below 97%. Phylogenetic analysis of the 16S rRNA gene sequences showed that strain YXT grouped with P. yonginense HMD1002T. The values of DNA–DNA hybridization and genomic orthologous average nucleotide identity (orthoANI) between strain YXT and Pedobacter yonginense KCTC 22721T were 58.5% and 82.0%, respectively. The genome size of strain YXT was 5.0 Mb, comprising 4369 predicted genes with a DNA G+C content of 37.3 mol %. Strain YXT had menaquinone-7 as the only respiratory quinone and polar lipids of phosphatidylethanolamine, sphingolipid, aminolipid and three unidentified lipids. The predominant cellular fatty acids (> 10%) of strain YXT were summed feature 3 (iso-C15:0 2-OH and/or C16:1ω7c), iso-C15:0 and iso-C17:0 3-OH. Strain YXT could be distinguished from the other Pedobacter members based on the data of phylogenetic distance, DNA–DNA hybridization, genomic orthoANI, RecA MLSA, core-protein comparison, and hydrolyses of l-arginine, utilization of d/l-lactate, l-alanine, 5-ketonic gluconate and glycogen. Therefore, strain YXT represents a novel species of the genus Pedobacter, for which the name Pedobacter paludis sp. nov. is proposed. The type strain is YXT (= KCTC 62520T = CCTCC AB 2018029T).
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页码:349 / 355
页数:6
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