Isolation and characterization of 16 polymorphic microsatellite markers for the mudskipper (Boleophthalmus pectinirostris)

被引:0
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作者
Cong-Tao Wang
Zhi-Zhi Liu
Ling-Bo Ma
Jia-Mei Zhang
Zhen Zeng
An-Yuan He
Shou-Jie Tang
Wen-Qiao Tang
机构
[1] Shanghai Ocean University,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education
[2] East China Sea Fishery Research Institute of Chinese Academy of Fishery Sciences,Key Laboratory of Freshwater Aquatic Germ Plasm Resources, Ministry of Agriculture
[3] Shanghai Ocean University,undefined
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关键词
Mudskipper; Microsatellite markers;
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摘要
The mudskipper, Boleophthalmus pectinirostris (Perciformes, Gobiidae), is an amphibious gobioid fish and a good species for marine culture in China. In this paper, 16 polymorphic microsatellite loci were isolated and characterized. The markers were tested on 60 individuals collected from Beihai (BH) and Lianyungang (LYG) coast in China. The number of alleles (NA) in these markers ranged from 5 to 11. The polymorphism information content varied from 0.5946 to 0.8567 in the two populations. The observed (HO) and expected (HE) heterozygosity ranged from 0.5333 to 0.9000 in BH and from 0.5333 to 0.8729 in LYG, respectively. No significant linkage disequilibrium was detected at any pair of loci (P > 0.01). These microsatellite loci will be useful for population genetic studies of B. pectinirostris.
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页码:1059 / 1061
页数:2
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