Live-Cell Super-resolution Fluorescence Microscopy

被引：0
|
作者：
A. S. Mishin
K. A. Lukyanov
机构：
[1] Russian Academy of Sciences,Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
来源：
Biochemistry (Moscow) | 2019年 / 84卷
关键词：
super-resolution fluorescence microscopy; STED; RESOLFT; single molecule localization; protein labeling; photoconversion;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Super-resolution fluorescence microscopy (nanoscopy) enables imaging with a spatial resolution much higher than the diffraction limit of optical microscopy. However, the methods of fluorescence nanoscopy are still poorly suitable for studying living cells. In this review, we describe some examples of live nanoscopy-based discoveries and focus on the development of methods for nanoscopy and specific fluorescent labeling aimed to decrease the damaging effects of light illumination on live samples.
引用
收藏
页码：19 / 31
页数：12
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