Rapid detection of food-borne Listeria monocytogenes by real-time quantitative loop-mediated isothermal amplification

被引：0
|
作者：
Xiaoxiao Shan
Yaoqi Zhang
Zhigang Zhang
Miaorui Chen
Yongyu Su
Yingna Yuan
M. Jahangir Alam
He Yan
Lei Shi
机构：
[1] South China University of Technology,College of Light Industry and Food Sciences
[2] South China University of Technology,School of Bioscience and Bioengineering
[3] Xiamen Yinxiang Group Co.,State Key Laboratory of Food Safety Technology for Meat Products
[4] Ltd.,College of Landscape and Art
[5] Jiangxi Agricultural University,undefined
[6] Texas Commission on Environmental Quality,undefined
来源：
Food Science and Biotechnology | 2012年 / 21卷
关键词：
loop-mediated isothermal amplication (LAMP); real-time turbidimeter; quantitative;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The purpose of this study was to develop a real-time quantitative loop-mediated isothermal amplication (LAMP) method for the rapid, sensitive, and convenient detection of Listeria monocytogenes in food. The LAMP method could amplify the hlyA gene of L. monocytogenes successfully at 63°C with a loopamp real-time turbidimeter. The detection limits of the LAMP for hlyA gene were 6 colony forming units (CFU)/tube. A standard curve was generated for L. monocytogenes LAMP by plotting the graph based different log CFU values of L. monocytogenes and time of positivity through real-time monitoring of the amplication. Then, the LAMP method was employed to test 94 retail food samples effectively. Sensitivity in detection of L. monocytogenes by the LAMP was higher than that of PCR and none of the conventional methodpositive samples was missed by the LAMP method.
引用
收藏
页码：101 / 106
页数：5
相关论文
共 50 条
  • [41] Rapid and real-time detection of Chikungunya virus by reverse transcription loop-mediated isothermal amplification assay
    Parida, M. M.
    Santhosh, S. R.
    Dash, P. K.
    Tripathi, N. K.
    Lakshmi, V.
    Mamidi, N.
    Shrivastva, A.
    Gupta, N.
    Saxena, P.
    Babu, J. Pradeep
    Rao, P. V. Lakshmana
    Morita, Kouichi
    JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (02) : 351 - 357
  • [42] Rapid detection of the common avian leukosis virus subgroups by real-time loop-mediated isothermal amplification
    Peng, Hao
    Qin, Lili
    Bi, Yuyu
    Wang, Peikun
    Zou, Guangzhen
    Li, Jun
    Yang, Yongli
    Zhong, Xingfu
    Wei, Ping
    VIROLOGY JOURNAL, 2015, 12
  • [43] Development of a Real-Time Loop-Mediated Isothermal Amplification Assay for the Rapid Detection of Olea Europaea Geminivirus
    Bertacca, Sofia
    Caruso, Andrea Giovanni
    Trippa, Daniela
    Marchese, Annalisa
    Giovino, Antonio
    Matic, Slavica
    Noris, Emanuela
    San Ambrosio, Maria Isabel Font
    Alfaro, Ana
    Panno, Stefano
    Davino, Salvatore
    PLANTS-BASEL, 2022, 11 (05):
  • [44] Loop-Mediated Isothermal Amplification: Rapid Visual and Real-Time Methods for Detection of Genetically Modified Crops
    Randhawa, Gurinder Jit
    Singh, Monika
    Morisset, Dany
    Sood, Payal
    Zel, Jana
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2013, 61 (47) : 11338 - 11346
  • [45] Real-time loop-mediated isothermal amplification assay for rapid and accurate detection of fungal pathogens of wheat
    Gomez, S. V.
    Goodwin, S. B.
    PHYTOPATHOLOGY, 2020, 110 (12) : 101 - 101
  • [46] Development and evaluation of real-time loop-mediated isothermal amplification assay for rapid detection of cystic echinococcosis
    Ahmed, Mohamed E.
    Eldigail, Mawahib H.
    Elamin, Fatima M.
    Ali, Ibtisam A.
    Grobusch, Martin P.
    Aradaib, Imadeldin E.
    BMC VETERINARY RESEARCH, 2016, 12
  • [47] Development and evaluation of real-time loop-mediated isothermal amplification assay for rapid detection of cystic echinococcosis
    Mohamed E. Ahmed
    Mawahib H. Eldigail
    Fatima M. Elamin
    Ibtisam A. Ali
    Martin P. Grobusch
    Imadeldin E. Aradaib
    BMC Veterinary Research, 12
  • [48] Rapid and real-time detection of hepatitis A virus by reverse transcription loop-mediated isothermal amplification assay
    Yoneyama, Tetsuo
    Kiyohara, Tomoko
    Shimasaki, Noriko
    Kobayashi, Gen
    Ota, Yoshinori
    Notomi, Tsugunori
    Totsuka, Atsuko
    Wakita, Takaji
    JOURNAL OF VIROLOGICAL METHODS, 2007, 145 (02) : 162 - 168
  • [49] Rapid and real-time detection of Porcine Sapelovirus by reverse transcription loop-mediated isothermal amplification assay
    Wang, Chunyan
    Yu, Dayi
    Cui, Li
    Hua, Xiuguo
    Yuan, Congli
    Sun, Huan
    Liu, Yuxiao
    JOURNAL OF VIROLOGICAL METHODS, 2014, 203 : 5 - 8
  • [50] Rapid detection of the common avian leukosis virus subgroups by real-time loop-mediated isothermal amplification
    Hao Peng
    Lili Qin
    Yuyu Bi
    Peikun Wang
    Guangzhen Zou
    Jun Li
    Yongli Yang
    Xingfu Zhong
    Ping Wei
    Virology Journal, 12
12345