A preclinical large-animal model for the assessment of critical-size load-bearing bone defect reconstruction

被引:0
|
作者
David S. Sparks
Siamak Saifzadeh
Flavia Medeiros Savi
Constantin E. Dlaska
Arne Berner
Jan Henkel
Johannes C. Reichert
Martin Wullschleger
Jiongyu Ren
Amaia Cipitria
Jacqui A. McGovern
Roland Steck
Michael Wagels
Maria Ann Woodruff
Michael A. Schuetz
Dietmar W. Hutmacher
机构
[1] Queensland University of Technology,Centre in Regenerative Medicine, Institute of Health and Biomedical Innovation
[2] Princess Alexandra Hospital,Department of Plastic & Reconswrapping a sterile Coban wrap around the limb distallytructive Surgery
[3] University of Queensland,Southside Clinical Division, School of Medicine
[4] Queensland UCoban wrap only comes non-sterile. Sterilize Coban wrap before use.niversity of Technology,Medical Engineering Research Facility
[5] Queensland University of Technology,ARC Centre for Additive Biomanufactthe mounting resin base cement. Use it only in a laboratory fume cabinet and withuring
[6] Royal Brisbane Hospital,Jamieson Trauma Institute
[7] University Hospital of Regensburg,Department of Trauma Surgery
[8] Julius-Maximilians-University,Department of Orthopaedic Surgery, Center for Musculoskeletal Research, König
[9] Evangelisches Waldkrankenhaus Spandau,Ludwig
[10] Griffith University,Haus
[11] School of Medicine,Department of Orthopaedic and Trauma Surgery
[12] Max Planck Institute of Colloids and Interfaces,Department of Biomaterials
[13] Princess Alexandra Hospital,Australian Centre for Complex Integrated Surgical Solutions (ACCISS)
[14] Institute of Health and Biomedical Innovation,Biofabrication and Tissue Morphology Group
[15] Queensland University of Technology,undefined
来源
Nature Protocols | 2020年 / 15卷
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摘要
Critical-size bone defects, which require large-volume tissue reconstruction, remain a clinical challenge. Bone engineering has the potential to provide new treatment concepts, yet clinical translation requires anatomically and physiologically relevant preclinical models. The ovine critical-size long-bone defect model has been validated in numerous studies as a preclinical tool for evaluating both conventional and novel bone-engineering concepts. With sufficient training and experience in large-animal studies, it is a technically feasible procedure with a high level of reproducibility when appropriate preoperative and postoperative management protocols are followed. The model can be established by following a procedure that includes the following stages: (i) preoperative planning and preparation, (ii) the surgical approach, (iii) postoperative management, and (iv) postmortem analysis. Using this model, full results for peer-reviewed publication can be attained within 2 years. In this protocol, we comprehensively describe how to establish proficiency using the preclinical model for the evaluation of a range of bone defect reconstruction options.
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页码:877 / 924
页数:47
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