Plant regeneration from embryo-derived callus in Phaseolus vulgaris L. (common bean) and P. acutifolius A. Gray (tepary bean)

被引：0

作者：

M. A. Zambre

J. De Clercq

E. Vranová

M. Van Montagu

G. Angenon

W. Dillen

机构：

[1] Laboratorium voor Genetica,

[2] Departement Genetica,undefined

[3] Vlaams Interuniversitair Instituut voor Biotechnologie,undefined

[4] Universiteit Gent,undefined

[5] K. L. Ledeganckstraat 35,undefined

[6] B-9000 Gent,undefined

[7] Belgium Fax: +32-9-2645349 E-mail: mamon@gengenp.rug.ac.be,undefined

来源：

Plant Cell Reports | 1998年 / 17卷

关键词：

Key words Fabaceae; In vitro regeneration; In vitro grafting; Legumes; Organogenesis;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Regeneration-competent callus of Phaseolus vulgaris and P. acutifolius was obtained from mature embryo explants on a medium containing thidiazuron and indole-3-acetic acid. For the P. vulgaris genotype Xan-159, regeneration was achieved from cotyledon explants, but not from embryonic axis explants. Both explants could be used for the P. acutifolius genotype NI 574 but embryonic axes gave the best results. In-vitro-rooted plantlets of P. acutifolius could readily be established in the greenhouse. For P. vulgaris hardening problems with in-vitro-rooted plantlets could be overcome by means of in vitro grafting. The potential of the described procedure for obtaining transgenic P. vulgaris plants is discussed.

引用

页码：626 / 630

页数：4

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