Engineering tolerance and accumulation of lead and cadmium in transgenic plants

被引:0
|
作者
Won-Yong Song
Eun Ju Sohn
Enrico Martinoia
Yong Jik Lee
Young-Yell Yang
Michal Jasinski
Cyrille Forestier
Inwhan Hwang
Youngsook Lee
机构
[1] National Research Laboratory for Phytoremediation,Division of Molecular Life Sciences
[2] Center for Plant Intracellular Trafficking,undefined
[3] POSTECH,undefined
[4] Institut für Pflanzenbiologie,undefined
[5] Universität Zürich,undefined
[6] CEA Cadarache,undefined
[7] DEVM-LEMS,undefined
[8] UMR 163 CEA-CNRS,undefined
来源
Nature Biotechnology | 2003年 / 21卷
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摘要
We have studied the utility of the yeast protein YCF1, which detoxifies cadmium by transporting it into vacuoles, for the remediation of lead and cadmium contamination. We found that the yeast YCF1-deletion mutant DTY167 was hypersensitive to Pb(II) as compared with wild-type yeast. DTY167 cells overexpressing YCF1 were more resistant to Pb(II) and Cd(II) than were wild-type cells, and accumulated more lead and cadmium. Analysis of transgenic Arabidopsis thaliana plants overexpressing YCF1 showed that YCF1 is functionally active and that the plants have enhanced tolerance of Pb(II) and Cd(II) and accumulated greater amounts of these metals. These results suggest that transgenic plants expressing YCF1 may be useful for phytoremediation of lead and cadmium.
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页码:914 / 919
页数:5
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