The role of the megagametophyte in maintaining loblolly pine (Pinus taeda L.) seedling arginase gene expression in vitro

被引:0
|
作者
Christopher D. Todd
David J. Gifford
机构
[1] Department of Biological Sciences,
[2] University of Alberta,undefined
[3] Edmonton,undefined
[4] Alberta,undefined
[5] T6G 2E9,undefined
[6] Canada,undefined
来源
Planta | 2002年 / 215卷
关键词
Arginine Arginase Pinus (arginase) Seedling development Tissue culture (loblolly pine);
D O I
暂无
中图分类号
学科分类号
摘要
Following loblolly pine (Pinus taeda L.) seed germination, storage-protein breakdown in the megagametophyte and in the seedling results in a large increase in the seedling's free amino acid pool. A substantial portion of both the storage proteins and the amino acid pool is arginine, a very efficient nitrogen-storage compound. Free arginine is hydrolyzed in the seedling by the enzyme arginase (EC 3.5.3.1), which is under strong developmental control. At present, regulation of arginase in conifers is not well understood. Here we report the utilization of an in vitro culture system to address the separate impacts of the seedling and megagametophyte tissues on arginase enzyme activity, protein levels and patterns of gene expression. We also describe the generation of an anti-arginase antibody prepared from a histidine-tagged loblolly pine arginase fusion protein expressed in Escherichia coli. Our results indicate that arginase gene expression in the seedling is initiated by the seedling itself and then maintained or up-regulated by the megagametophyte. The contribution of storage-protein breakdown and the free amino acid pool, particularly arginine, in this regulation is also addressed.
引用
收藏
页码:110 / 118
页数:8
相关论文
共 50 条
  • [31] MicroRNAs in loblolly pine (Pinus taeda L.) and their association with fusiform rust gall development
    Lu, Shanfa
    Sun, Ying-Hsuan
    Amerson, Henry
    Chiang, Vincent L.
    PLANT JOURNAL, 2007, 51 (06): : 1077 - 1098
  • [32] Response of densely stocked loblolly pine (Pinus taeda L.) to applied nitrogen and phosphorus
    Bekele, A. (abekele@tiaer.tarleton.edu), 1600, Society of American Foresters (27):
  • [33] Modeling thinning effects on ring specific gravity of loblolly pine (Pinus taeda L.)
    Tasissa, G
    Burkhart, HE
    FOREST SCIENCE, 1998, 44 (02) : 212 - 223
  • [34] The effects of drought and disturbance on the growth and developmental instability of loblolly pine (Pinus taeda L.)
    Graham, John H.
    Duda, Jeffrey J.
    Brown, Michelle L.
    Kitchen, Stanley
    Emlen, John M.
    Malol, Jagadish
    Bankstahl, Elizabeth
    Krzysik, Anthony J.
    Balbach, Harold
    Freeman, D. Carl
    ECOLOGICAL INDICATORS, 2012, 20 : 143 - 150
  • [35] Influence of spacing regimes on the development of loblolly pine (Pinus taeda L.) in Southern Brazil
    Cardoso, Denise Jeton
    Biscaia Lacerda, Andre Eduardo
    Doetzer Rosot, Maria Augusta
    Garrastazu, Marilice Cordeiro
    Lima, Renato Teixeira
    FOREST ECOLOGY AND MANAGEMENT, 2013, 310 : 761 - 769
  • [36] Root Nonstructural Carbohydrates and Their Relationship with Autotrophic Respiration of Loblolly Pine (Pinus taeda L.)
    Heim, Brett C.
    Seiler, John R.
    Strahm, Brian D.
    COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS, 2015, 46 (07) : 904 - 912
  • [37] Seasonal charges in water source of four families of loblolly pine (Pinus taeda L.)
    Retzlaff, WA
    Blaisdell, GK
    Topa, MA
    TREES-STRUCTURE AND FUNCTION, 2001, 15 (03): : 154 - 162
  • [38] Influence of thinning loblolly pine (Pinus taeda L.) on hydraulic properties of an organic soil
    Grace, J. M., III
    Skaggs, R. W.
    Cassel, D. K.
    TRANSACTIONS OF THE ASABE, 2007, 50 (02) : 517 - 522
  • [39] The effect of storage time and moisture content on grindability of loblolly pine (Pinus taeda L.)
    Oyedeji, O.
    Fasina, O.
    Adhikari, S.
    McDonald, T.
    Taylor, S.
    EUROPEAN JOURNAL OF WOOD AND WOOD PRODUCTS, 2016, 74 (06) : 857 - 866
  • [40] The effect of storage time and moisture content on grindability of loblolly pine (Pinus taeda L.)
    O. Oyedeji
    O. Fasina
    S. Adhikari
    T. McDonald
    S. Taylor
    European Journal of Wood and Wood Products, 2016, 74 : 857 - 866