Fluorescence resonance energy transfer (FRET) as a method to calculate the dimerization strength of basic Helix-loop-Helix (bHLH) proteins

被引：13

作者：

Centonze V.E. ^{[1
]}

Firulli B.A. ^{[2
]}

Firulli A.B. ^{[2
]}

机构：

[1] Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, TX

[2] Wells Center for Pediatric Research, James Whitcomb Riley Hospital for Children, Indianapolis University Medical School, Indianapolis, IN 46202-5225

来源：

Biological Procedures Online | 2004年 / 6卷 / 1期

关键词：

Fluorescence resonance energy transfer; Phosphorylation; Transcription factors;

D O I：

10.1251/bpo75

中图分类号：

学科分类号：

摘要：

Post-translational modifications such as phosphorylation play a vital role in the regulation of protein function. In our study of the basic Helix-loop-Helix (bHLH) transcription factor HAND1, we show that HAND1 is phosphorylated during the trophoblast giant cell differentiation on residues residing in Helix I of the bHLH domain. Our hypothesis is that these modifications result in changes in HAND1 dimerization affinities with other bHLH factors. To test this idea, we employed FRET to measure the protein-protein interactions of HAND1 and HAND1 point mutants in HEK293 cells using YFP and CFP fusion proteins and laser scanning confocal microscopy. © 2004. Biological Procedures Online.

引用

页码：78 / 82

页数：4

共 50 条

[41] CLONING OF NOVEL BASIC HELIX-LOOP-HELIX PROTEINS EXPRESSED DURING EARLY CARDIAC DEVELOPMENT
SRIVASTAVA, D
CSERJESI, P
OLSON, EN
CIRCULATION, 1994, 90 (04) : 78 - 78
[42] Basic helix loop helix (bHLH) proteins regulate cfos expression in differentiating Sertoli cells.
Chaudhary, J
Patel, U
Skinner, MK
BIOLOGY OF REPRODUCTION, 1997, 56 : 76 - 76
[43] Id2 promotes apoptosis by a novel mechanism independent of dimerization to basic helix-loop-helix factors
Florio, M
Hernandez, MC
Yang, H
Shu, HK
Cleveland, JL
Israel, MA
MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (09) : 5435 - 5444
[44] A novel basic helix-loop-helix (bHLH) transcriptional repressor, NeuroAB, expressed in bipolar and amacrine cells in the chick retina
Ohkawara, T
Shintani, T
Saegusa, C
Yuasa-Kawada, J
Takahashi, M
Noda, M
MOLECULAR BRAIN RESEARCH, 2004, 128 (01): : 58 - 74
[45] Identification and characterization of a putative basic helix-loop-helix (bHLH) transcription factor interacting with calcineurin in C. elegans
Lee, Soo-Ung
Song, Hyun-Ok
Lee, Wonhae
Singaravelu, Gunasekaran
Yu, Jae-Ran
Park, Woo-Yoon
MOLECULES AND CELLS, 2009, 28 (05) : 455 - 461
[46] Genomic Characterization and Expression Analysis of Basic Helix-Loop-Helix (bHLH) Family Genes in Traditional Chinese Herb Dendrobium officinale
Wang, Yue
Liu, Aizhong
PLANTS-BASEL, 2020, 9 (08): : 1 - 23
[47] Genome-Wide Identification and Expression Analysis of the Basic Helix-Loop-Helix (bHLH) Transcription Factor Family in Gastrodia elata
Lan, Shanshan
Lin, Xin
Wei, Maoqiong
Feng, Guangheng
Liu, Hongcheng
Shao, Jinliang
Li, Xinyu
Chen, Zeli
Liu, Zhenhuan
PLANT MOLECULAR BIOLOGY REPORTER, 2025,
[48] Functional characterization of a basic helix-loop-helix (bHLH) transcription factor GhDEL65 from cotton (Gossypium hirsutum)
Shangguan, Xiao-Xia
Yang, Chang-Qing
Zhang, Xiu-Fang
Wang, Ling-Jian
PHYSIOLOGIA PLANTARUM, 2016, 158 (02) : 200 - 212
[49] Highly conserved sequences mediate the dynamic interplay of basic helix-loop-helix proteins regulating retinogenesis
Hernandez, Julio
Matter-Sadzinski, Lidia
Skowronska-Krawczyk, Dorota
Chiodini, Florence
Alliod, Christine
Ballivet, Marc
Matter, Jean-Marc
JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (52) : 37894 - 37905
[50] Genome-Wide Identification of Basic Helix-Loop-Helix (bHLH) Family in Peanut: Potential Regulatory Roles in Iron Homeostasis
Shi, Gangrong
Zhang, Zheng
Li, Jinxiu
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2024, 25 (22)

← 1 2 3 4 5 →