Characterization of a confocal microscope for time-resolved photon counting fluorescence

被引：0
|
作者：
Schoutteten L. [1 ]
Denjean P. [1 ]
Pansu R.B. [1 ]
机构：
[1] ENS Cachan, PPSM (Photophysique et Photochimie Supramoléculaires et Macromoléculaires), URA 1906 CNRS, 94235 Cachan Cedex
来源：
Journal of Fluorescence | 1997年 / 7卷 / 2期
关键词：
Anisotropy; Calcium probes; Confocal microscopy; Fluorescence correlation spectroscopy; Laser; Time-resolved fluorescence;
D O I：
10.1007/BF02760507
中图分类号：
学科分类号：
摘要：
A confocal microscope setup is developed for time-resolved fluorescence measurements. It is added to a traditional cuvette time-resolved setup, with a pumped Ti-Sa light source. The temporal resolution of 37 ps (FWHM) is not degraded, in comparison with the cuvette setup also described. These setups allow both decay lifetime and anisotropy relaxation time determination. Fluorescence correlation spectroscopy (FCS) is used to determine the observation point size. When associated with the calcium probe calcium green, calcium concentration in single cells can be determined in 10 ms by simultaneous acquisition of early and late fluorescence photons. © 1997 Plenum Publishing Corporation.
引用
收藏
页码：155 / 165
页数：10
相关论文
共 50 条
  • [41] Time-resolved 3D confocal fluorescence microscopy on living cells
    Schellenberg, M.
    Peev, E.
    Kloster, M.
    Napier, J.
    Neu, W.
    [J]. LASER APPLICATIONS IN LIFE SCIENCES, 2010, 7376
  • [42] Fluorescence Dynamics in the Endoplasmic Reticulum of a Live Cell: Time-Resolved Confocal Microscopy
    Ghosh, Shirsendu
    Nandi, Somen
    Ghosh, Catherine
    Bhattacharyya, Kankan
    [J]. CHEMPHYSCHEM, 2016, 17 (18) : 2818 - 2823
  • [43] Time-resolved fluorescence decay analysis of photoresist film by GaN LD pumped single-photon counting method
    Kaji, Makoto
    Muramatsu, Yukiko
    Nishimura, Yoshinobu
    Arai, Tatsuo
    [J]. JOURNAL OF PHOTOPOLYMER SCIENCE AND TECHNOLOGY, 2007, 20 (02) : 265 - 269
  • [44] Molecular Counting by Photon Statistics in Confocal Fluorescence Imaging
    Koenig, Marcelle
    Berlage, Caroline
    Reisch, Paja
    Oelsner, Christian
    Koberling, Felix
    Ta, Haisen
    Erdmann, Rainer
    [J]. BIOPHYSICAL JOURNAL, 2019, 116 (03) : 134A - 135A
  • [45] Two-photon excitation and time-resolved fluorescence:: 1.: The proper response function for analysing single-photon counting experiments
    Habenicht, A
    Hjelm, J
    Mukhtar, E
    Bergström, F
    Johansson, LBÅ
    [J]. CHEMICAL PHYSICS LETTERS, 2002, 354 (5-6) : 367 - 375
  • [46] SPAD Image Sensor With Analog Counting Pixel for Time-Resolved Fluorescence Detection
    Pancheri, Lucio
    Massari, Nicola
    Stoppa, David
    [J]. IEEE TRANSACTIONS ON ELECTRON DEVICES, 2013, 60 (10) : 3442 - 3449
  • [47] Time-correlated photon counting instrumentation for time-resolved photoluminescence measurement on semiconductor surfaces
    Wahl, M
    [J]. ULTRAFAST PHENOMENA IN SEMICONDUCTORS V, 2001, 4280 : 166 - 173
  • [48] Image quality in time-resolved laser transillumination by time-correlated single photon counting
    Daniele, A.
    Mormile, M.
    Esposito, R.
    Lepore, A.
    Indovina, P. L.
    [J]. BIOPHOTONICS AND NEW THERAPY FRONTIERS, 2006, 6191
  • [49] Phase estimation of time-bin qudits by time-resolved single-photon counting
    Czerwinski, Artur
    Sedziak-Kacprowicz, Karolina
    Kolenderski, Piotr
    [J]. PHYSICAL REVIEW A, 2021, 103 (04)
  • [50] The design of time-resolved electron microscope
    Li, SH
    Niu, H
    [J]. 22ND INTERNATIONAL CONGRESS ON HIGH-SPEED PHOTOGRAPHY AND PHOTONICS, 1997, 2869 : 222 - 228
12345