Characterization of a confocal microscope for time-resolved photon counting fluorescence

被引：0
|
作者：
Schoutteten L. [1 ]
Denjean P. [1 ]
Pansu R.B. [1 ]
机构：
[1] ENS Cachan, PPSM (Photophysique et Photochimie Supramoléculaires et Macromoléculaires), URA 1906 CNRS, 94235 Cachan Cedex
来源：
Journal of Fluorescence | 1997年 / 7卷 / 2期
关键词：
Anisotropy; Calcium probes; Confocal microscopy; Fluorescence correlation spectroscopy; Laser; Time-resolved fluorescence;
D O I：
10.1007/BF02760507
中图分类号：
学科分类号：
摘要：
A confocal microscope setup is developed for time-resolved fluorescence measurements. It is added to a traditional cuvette time-resolved setup, with a pumped Ti-Sa light source. The temporal resolution of 37 ps (FWHM) is not degraded, in comparison with the cuvette setup also described. These setups allow both decay lifetime and anisotropy relaxation time determination. Fluorescence correlation spectroscopy (FCS) is used to determine the observation point size. When associated with the calcium probe calcium green, calcium concentration in single cells can be determined in 10 ms by simultaneous acquisition of early and late fluorescence photons. © 1997 Plenum Publishing Corporation.
引用
收藏
页码：155 / 165
页数：10
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