Methodological Approaches to Standardization of Rhizomes and Roots of Eleutherococcus senticosus

Methodological approaches to the analysis of biologically active compounds in rhizomes and roots of Eleutherococcus senticosus (Rupr. et Maxim.) Maxim. [syn. Acanthopanax senticosus (Rupr. et Maxim.) Harms] were substantiated. An HPLC method for assay of eleutheroside B (syringin) and a spectrophotometric method for determination of the total content of phenylpropanoids in terms of eleutheroside B (syringin) isolated from medicinal plant raw material after preliminary purification and filtration through a layer of aluminum oxide were developed and validated. The total content of phenylpropanoids in roots and rhizomes of E. senticosus was found to vary from 0.30 ± 0.02% to 0.37 ± 0.02%; eleutheroside B, from 0.041 ± 0.002% to 0.089 ± 0.003%. Results of statistical processing indicated that the relative error of determining the mean total of phenylpropanoids and eleutheroside B (syringin) did not exceed ±5.0% at a confidence level of 95%.