Molecular cloning and expression analysis of pig CD7

被引：0
|
作者：
Ju Yeon Lee
Joonbeom Bae
Inho Choi
Chung-Gyu Park
Taehoon Chun
机构：
[1] Korea University,Division of Biotechnology, School of Life Sciences and Biotechnology
[2] Yeungnam University,School of Biotechnology
[3] Seoul National University College of Medicine,Department of Microbiology and Immunology
来源：
Veterinary Research Communications | 2014年 / 38卷
关键词：
CD7; cDNA; Galectin-3; Pig; Soluble fusion protein;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
CD7 is an integral membrane protein which mediates an important signal to mediate the differentiation, activation, and regulation of some T cells and NK cells. However, only human and mouse CD7 have been identified and studied among mammalian species. In this study, we cloned pig CD7 cDNA and determined its complete cDNA sequence. Pig CD7 cDNA contained an open reading frame (627 bp) encoding 208 amino acids with well conserved motifs involved in signal transduction within cytoplasmic tail among mammalian species. Pig CD7 mRNA was detected by RT-PCR in mainly lymphoid tissues, indicating the conserved functions of CD7 in pigs. Moreover, we generated soluble pig CD7 fusion immunoglobulin (pig CD7Ig) containing extracellular domain of pig CD7 to test whether pig CD7 binds to pig galectin-3. Flow cytometry and immunohistochemistry analyses indicated that soluble pig CD7Ig can bind to galectin-3 expressed in macrophages and epithelial cells of small intestine. These results help to analyze the structural relationship between CD7 and its ligand transferring signal transduction among mammalian species.
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页码：257 / 263
页数：6
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