Study of the oligomeric structure and some physicochemical properties of inulinase from Kluyveromyces marxianus Y-303

被引：2
|
作者：
Artyukhov V.G. [1 ]
Kovaleva T.A. [1 ]
Kholyavka M.G. [1 ]
Bityutskaya L.A. [1 ]
Grechkina M.V. [1 ]
Obraztsova T.B. [1 ]
机构：
[1] Voronezh State University
来源：
Biophysics | 2009年 / 54卷 / 6期
关键词：
Atomic force microscopy; Gel chromatography; Infrared spectroscopy; Inulinase; Oligomeric structure;
D O I：
10.1134/S0006350909060025
中图分类号：
学科分类号：
摘要：
It has been found using a combination of atomic force microscopy with infrared spectroscopy, gel chromatography, and electrophoresis that inulinase from Kluyveromyces marxianus Y-303 has oligomeric structure, which includes two subunits differing in size, molecular mass, and catalytic activity. It has been shown that the division of the inulinase dimer into monomers leads to an increase in the number of irregular regions by 6% for subunit 1 (54.8 kDa) and by 10% for subunit 2 (8.4 kDa) compared with the native enzyme. © Pleiades Publishing, Ltd 2009.
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收藏
页码：675 / 680
页数：5
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