Secretion of the sweet-tasting protein thaumatin by recombinant strains of Aspergillus niger var. awamori

被引：0

作者：

I. Faus

C. del Moral

N. Adroer

J. L. del Río

C. Patiño

H. Sisniega

C. Casas

J. Bladé

V. Rubio

机构：

[1] URQUIMA,

[2] S.A. (URIACH Group),undefined

[3] Arnau de Vilanova 22–42,undefined

[4] E-08015 Sant Fost de Campsentelles (Barcelona),undefined

[5] Spain Tel.: +34 3 570 5450 Fax: +34 3 570 2476,undefined

[6] Centro Nacional de Biotecnología (CSIC-UAM),undefined

[7] Campus Canto Blanco,undefined

[8] E-28049 Madrid,undefined

[9] Spain,undefined

[10] Unitat d'Enginyería Química,undefined

[11] Facultat de Ciencies,undefined

[12] Universitat Autónoma de Barcelona,undefined

[13] 08193 Bellaterra (Barcelona),undefined

[14] Spain,undefined

来源：

Applied Microbiology and Biotechnology | 1998年 / 49卷

关键词：

Aspergillus; Aspergillus Niger; Filamentous Fungus; Shake Flask; Fungal Strain;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A recombinant form of the sweet-tasting protein thaumatin has been produced in the filamentous fungus Aspergillus niger var. awamori. Expression cassettes containing a synthetic gene encoding thaumatin II were prepared and used to transform Aspergillus niger var. awamori strain NRRL312. Several fungal strains capable of synthesizing and secreting thaumatin into the culture medium were generated, and their production capabilities were determined, first in shake flasks and later in a laboratory fermentor. We report the expression and secretion of thaumatin in concentrations of 5–7 mg/l. This recombinant thaumatin is sweet.

引用

页码：393 / 398

页数：5

共 49 条

[41] Atomic structure of the sweet-tasting protein thaumatin I at pH 8.0 reveals the large disulfide-rich region in domain II to be sensitive to a pH change
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[42] Five amino acid residues in cysteine-rich domain of human T1R3 were involved in the response for sweet-tasting protein, thaumatin
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[43] Recombinant expression of the N-terminal domain of human T1R2 taste receptor: interaction with brazzein, a sweet-tasting protein
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[44] Production of GFP and glucoamylase by recombinant Aspergillus niger:: Effects of fermentation conditions on fungal morphology and protein secretion
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[45] Heterologous protein secretion and fungal morphology in chemostat cultures of a recombinant Aspergillus niger (B1-D)
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不详
Enzyme Microb. Technol., 8-9 (489-497):
[46] Heterologous protein secretion and fungal morphology in chemostat cultures of a recombinant Aspergillus niger (B1-D)
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[47] The cysteine-rich domain of human T1R3 is necessary for the interaction between human T1R2-T1R3 sweet receptors and a sweet-tasting protein, thaumatin
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[48] Gastric digestion of the sweet-tasting plant protein thaumatin releases bitter peptides that reduce H. pylori induced pro-inflammatory IL-17A release via the TAS2R16 bitter taste receptor
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FOOD CHEMISTRY, 2024, 448
[49] Impact of alg3 gene deletion on growth, development, pigment production, protein secretion, and functions of recombinant Trichoderma reesei cellobiohydrolases in Aspergillus niger
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