Secretion of the sweet-tasting protein thaumatin by recombinant strains of Aspergillus niger var. awamori

被引：0

作者：

I. Faus

C. del Moral

N. Adroer

J. L. del Río

C. Patiño

H. Sisniega

C. Casas

J. Bladé

V. Rubio

机构：

[1] URQUIMA,

[2] S.A. (URIACH Group),undefined

[3] Arnau de Vilanova 22–42,undefined

[4] E-08015 Sant Fost de Campsentelles (Barcelona),undefined

[5] Spain Tel.: +34 3 570 5450 Fax: +34 3 570 2476,undefined

[6] Centro Nacional de Biotecnología (CSIC-UAM),undefined

[7] Campus Canto Blanco,undefined

[8] E-28049 Madrid,undefined

[9] Spain,undefined

[10] Unitat d'Enginyería Química,undefined

[11] Facultat de Ciencies,undefined

[12] Universitat Autónoma de Barcelona,undefined

[13] 08193 Bellaterra (Barcelona),undefined

[14] Spain,undefined

来源：

Applied Microbiology and Biotechnology | 1998年 / 49卷

关键词：

Aspergillus; Aspergillus Niger; Filamentous Fungus; Shake Flask; Fungal Strain;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A recombinant form of the sweet-tasting protein thaumatin has been produced in the filamentous fungus Aspergillus niger var. awamori. Expression cassettes containing a synthetic gene encoding thaumatin II were prepared and used to transform Aspergillus niger var. awamori strain NRRL312. Several fungal strains capable of synthesizing and secreting thaumatin into the culture medium were generated, and their production capabilities were determined, first in shake flasks and later in a laboratory fermentor. We report the expression and secretion of thaumatin in concentrations of 5–7 mg/l. This recombinant thaumatin is sweet.

引用

页码：393 / 398

页数：5

共 49 条

[1] Secretion of the sweet-tasting protein thaumatin by recombinant strains of Aspergillus niger var. awamori
Faus, I
del Moral, C
Adroer, N
del Río, JL
Patiño, C
Sisniega, H
Casas, C
Bladé, J
Rubio, V
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1998, 49 (04) : 393 - 398
[2] High-yield Secretion of the Recombinant Sweet-Tasting Protein Thaumatin I
Masuda, Tetsuya
Ide, Nobuyuki
Ohta, Keisuke
Kitabatake, Naofumi
FOOD SCIENCE AND TECHNOLOGY RESEARCH, 2010, 16 (06) : 585 - 592
[3] SECRETION OF THE SWEET-TASTING PLANT PROTEIN THAUMATIN BY BACILLUS-SUBTILIS
ILLINGWORTH, C
LARSON, G
HELLEKANT, G
BIOTECHNOLOGY LETTERS, 1988, 10 (08) : 587 - 592
[4] SECRETION OF THE SWEET-TASTING PLANT PROTEIN THAUMATIN BY STREPTOMYCES-LIVIDANS
ILLINGWORTH, C
LARSON, G
HELLEKANT, G
JOURNAL OF INDUSTRIAL MICROBIOLOGY, 1989, 4 (01): : 37 - 42
[5] High-resolution structure of the recombinant sweet-tasting protein thaumatin I
Masuda, Tetsuya
Ohta, Keisuke
Mikami, Bunzo
Kitabatake, Naofumi
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 : 652 - 658
[6] MICROBIAL SYNTHESIS OF THE SWEET-TASTING PLANT PROTEIN THAUMATIN
EDENS, L
VANDERWEL, H
TRENDS IN BIOTECHNOLOGY, 1985, 3 (03) : 61 - 64
[7] Crystal structure of the sweet-tasting protein thaumatin II at 1.27 Å
Masuda, Tetsuya
Ohta, Keisuke
Tani, Fumito
Mikami, Bunzo
Kitabatake, Naofumi
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2011, 410 (03) : 457 - 460
[8] POSSIBLE ACTIVE-SITE OF THE SWEET-TASTING PROTEIN THAUMATIN
SLOOTSTRA, JW
DEGEUS, P
HAAS, H
VERRIPS, CT
MELOEN, RH
CHEMICAL SENSES, 1995, 20 (05) : 535 - 543
[9] CLONING OF THE NATURAL GENE FOR THE SWEET-TASTING PLANT PROTEIN THAUMATIN
LEDEBOER, AM
VERRIPS, CT
DEKKER, BMM
GENE, 1984, 30 (1-3) : 23 - 32
[10] ASSIGNMENT OF THE DISULFIDE BONDS IN THE SWEET-TASTING PROTEIN THAUMATIN-I
VANDERWEL, H
IYENGAR, RB
VANBROUWERSHAVEN, J
VANWASSENAAR, PD
BEL, WJ
VANDEROUDERAA, FJG
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 144 (01): : 41 - 45

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