In conventional Micro-Air-Vehicle design inspired by insects, the periodical motion of flapping airfoil usually leads to generation of a periodical aerodynamic force. However, recent studies indicate that time courses of aerodynamic force and flow structure of a flapping airfoil may be non-periodical even though the airfoil undergoes a periodical motion. In this paper, a computational fluid dynamics analysis is employed to investigate the effects of some dimensionless variables, such as Reynolds number, plunging amplitude, advance ratio, and angle of attack, on the periodicity of the flow around a flapping airfoil. The governing equations in an inertial frame of reference are solved to obtain unsteady flow structure and aerodynamic behaviors of the airfoil. It is found in the results that the periodicity of the flow and aerodynamics is greatly dependent on Reynolds number and plunging amplitude. Under given conditions, the product of these two variables may be utilized as a criterion parameter to judge whether the time course of the flow is periodical or not. In addition, a new mechanism that accounts for the non-periodical flow is revealed to explain the flow of airfoil with pre-stall angle of attack.