Correlation between small-cell lung cancer serum protein/peptides determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and chemotherapy efficacy

被引：0
|
作者：
Li, Zhihua [1 ]
Chen, Junnan [1 ]
Xu, Bin [3 ]
Zhao, Wei [1 ]
Zha, Haoran [1 ]
Han, Yalin [1 ]
Shen, Wennan [1 ]
Dong, Yuemei [1 ]
Zhao, Nan [1 ]
Zhang, Manze [1 ]
He, Kun [3 ]
Li, Zhaoxia [1 ]
Liu, Xiaoqing [2 ]
机构：
[1] PLA Rocket Force Characterist Med Ctr, Dept Oncol, Beijing 100088, Peoples R China
[2] Peoples Liberat Army Gen Hosp, Med Ctr 5, Dept Oncol, Beijing 100071, Peoples R China
[3] Natl Ctr Biomed Anal, Beijing 100850, Peoples R China
来源：
CLINICAL PROTEOMICS | 2024年 / 21卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Small-cell lung cancer; Matrix assisted laser desorption ionization-time of flight-mass spectrometry; Proteomics; Chemotherapy; Prediction of efficacy; PROTEOMIC ANALYSIS; IDENTIFICATION; PROTEINS; TECHNOLOGY; METASTASES; PEPTIDOME; PLASMA;
D O I：
10.1186/s12014-024-09483-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Background Currently, no effective measures are available to predict the curative efficacy of small-cell lung cancer (SCLC) chemotherapy. We expect to develop a method for effectively predicting the SCLC chemotherapy efficacy and prognosis in clinical practice in order to offer more pertinent therapeutic protocols for individual patients. Methods We adopted matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and ClinPro Tools system to detect serum samples from 154 SCLC patients with different curative efficacy of standard chemotherapy and analyze the different peptides/proteins of SCLC patients to discover predictive tumor markers related to chemotherapy efficacy. Ten peptide/protein peaks were significantly different in the two groups. Results A genetic algorithm model consisting of four peptides/proteins was developed from the training group to separate patients with different chemotherapy efficacies. Among them, three peptides/proteins (m/z 3323.35, 6649.03 and 6451.08) showed high expression in the disease progression group, whereas the peptide/protein at m/z 4283.18 was highly expressed in the disease response group. The classifier exhibited an accuracy of 91.4% (53/58) in the validation group. The survival analysis showed that the median progression-free survival (PFS) of 30 SCLC patients in disease response group was 9.0 months; in 28 cases in disease progression group, the median PFS was 3.0 months, a statistically significant difference (chi 2 = 46.98, P < 0.001). The median overall survival (OS) of the two groups was 13.0 months and 7.0 months, a statistically significant difference (chi 2 = 40.64, P < 0.001). Conclusions These peptides/proteins may be used as potential biological markers for prediction of the curative efficacy and prognosis for SCLC patients treated with standard regimen chemotherapy.
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页数：10
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